Locus-specific EPIC-PCR primers for four distinct calmodulin genes of the Pacific bluefin tuna Thunnus orientalis (Temminck & Schlegel, 1844)

Locus-specific EPIC-PCR primers for four distinct calmodulin genes of the Pacific bluefin tuna Thunnus orientalis (Temminck & Schlegel, 1844)

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Abstract Nucleotide sequences of four distinct calmodulin genes (designated as CaM-A to -D) of the Pacific bluefin tuna (Thunnus orientalis) were compared.Nucleotide sequence divergence between earrings the coding regions of four genes ranged from 16.0 to 21.

6%, and locus-specific eight primer pairs targeting introns were designed.These primer pairs amplified expected size of fragments in five congeneric tuna species, and utility of these primer pairs was Air Intake Systems investigated using distant fish species.